Manfred Lindau
Professor

Manfred Lindau

Phone

607-255-5264

Address

School of Applied Engineering Physics
217 Clark Hall
Cornell University
Ithaca, NY 14853-2703

Email

Web Sites

Lab Web Site
Department Profile

Background

Dipl. 1977 (University of Hamburg, Germany: physics),
Dr. rer. nat. 1983 (Technical University of Berlin: chemistry),
Habil. 1991 (Free University of Berlin: experimental physics)

Research Description

  • Characterization of single exocytotic events in cell: attached patches using   
  • capacitance measurements and amperometry
  • Spatio-temporal dynamics of exocytosis using nanofabricated electrochemical detector arrays
  • Reconstitution of exocytosis in excised membrane patches
  • Direct visualization of the degranulation process in white blood cells using modern microscopic imaging methods
  • Characterization of docking and fusion between secretory granules and between specific components of the exocytotic machinery using optical tweezers

Publications

  • Scepek, S., and M. Lindau. 1993. Focal exocytosis by eosinophils: Compound exocytosis and cumulative fusion. EMBO Journal 12:1811-17.

  • Rosenboom, H., and M. Lindau. 1994. Exo-endocytosis and closing of the fission pore during endocytosis in single pituitary nerve terminals internally perfused with high calcium concentrations. Proceedings of the National Academy of Sciences, USA 91:5267-71.

  • Lollike, K., N. Borregaard, and M. Lindau. 1995. The exocytotic fusion pore of small granules has a   conductance similar to an ion channel. Journal of Cellular Biology 129:99-104.

  • Albillos, A., G. Dernick, H. Horstmann, W. Almers, G. Alvarez de Toledo, and M. Lindau. 1997. The exocytotic event in chromaffin cells revealed by patch amperometry. Nature 389:509-12.

  • Ales, E., L. Tabares, F. N. Poyato, V. Valero, M. Lindau, and G. Alvarez de Toledo. 1999. High calcium concentrations shift the mode of exocytosis to the kiss-and-run mechanism. Nature Cell Biology 1:40- 44.

  • Dias A.F., Dernick, G., Valero, V., Yong, M.G., James, C.D., Craighead, H.G. & Lindau, M. An electrochemical detector array to study cell biology on the nanoscale. Nanotechnology 13, 285-289 (2002).

  • Dernick G., Alvarez de Toledo, G. & Lindau, M. Exocytosis of single chromaffin granules in cell-free inside-out membrane patches. Nat Cell Biol 5, 358-62 (2003).

  • Lindau M. & Alvarez de Toledo, G. The fusion pore. Biochim Biophys Acta 1641, 167-173 (2003).

    Hartmann J., Scepek, S., Hafez, I. & Lindau, M. Differential regulation of exocytotic fusion and granule- granule fusion in eosinophils by Ca2+ and GTP analogs. J Biol Chem (2003).

  • Hafez I., Stolpe, A. & Lindau, M. Compound exocytosis and cumulative fusion in eosinophils. J Biol Chem 278, 44921-8 (2003).

  • Gong L.W., Hafez, I., Alvarez de Toledo, G. & Lindau, M. Secretory vesicles membrane area is regulated in tandem with quantal size in chromaffin cells. J Neurosci 23, 7917-21 (2003).

Click here for a full list of publications.