Yimon Aye
Assistant Professor

Yimon Aye

Phone

607-254-4519

Address

Department of Chemistry & Chemical Biology
Cornell University
224A Baker Laboratory
Ithaca, NY 14853

Email

Web Sites

Lab Web Site
Department Profile

Background

Yimon Aye received a B.S. degree in 2003 and a Master’s degree in 2004 in Chemistry from the University of Oxford, and a Ph.D. in Organic Chemistry from Harvard University in 2009.  She completed her postdoctoral training in Cancer Biochemistry at MIT. She became an Assistant Professor in the Department of Chemistry & Chemical Biology at Cornell University in July 2012.

Research Description

The Aye group is working on important biological problems in a multidisciplinary setting in the realms of method development in chemical biology, mechanistic biochemistry, biophysics, and mammalian cell biology.

Specific Aims:

1) We are exploiting the target-specific redox perturbation platform that our lab has recently developed to deconstruct individual oxidative and electrophilic signaling events in living cells and animals..

2) We are seeking to advance mechanistic understanding of small-molecule-driven protein oligomeric regulation through approaches that combine in vitro studies, cell culture and mouse models.

3) We are investigating the mechanism and regulation of redox-dependent metalloenzymes in isolated systems and in intact mammalian cells.

4) We are developing a new chemical platform for manipulating intracellular protein trafficking.

Selected Publications

Lin, H.-Y., Haegele, J.A., Disare, M.T., Lin, Q., and Aye, Y. (2015) A generalizable platform for interrogating target- and signal-specific consequence of electrophilic modifications in redox-dependent cell signaling.  J. Amer. Chem. Soc. 137, 6232-6244.

Parvez, S., Fu, Y., Li, J., Long, M.J.C., Lin H.-Y., Lee, D.K., Hu, G.S., and Aye, Y. (2015) Substoichiometric hydroxynonenylation of a single protein recapitulates whole-cell-stimulated antioxidant response.  J. Amer. Chem. Soc. 137, 10-13.

Aye, Y., Li, M., Long, M.J., and Weiss, R.S. (2015) Ribonucleotide reductase and cancer: biological mechanisms and targeted therapies.  Oncogene 34, 2011-2021.

Fang, X., Fu, Y., Long, M.J.C., Haegele, J.A., Ge, E.J., Parvez, S., and Aye, Y. (2013) Temporally controlled targeting of 4-hydroxynonenal to specific proteins in living cells.  J. Amer. Chem. Soc. 135, 14496-9.

Fu, Y., Long, M.J.C., Rigney, M., Parvez, S., Blessing, W.A., and Aye, Y. (2013) Uncoupling of allosteric and oligomeric regulation in a functional hybrid enzyme constructed from Escherichia coli and human ribonucleotide reductase.  Biochemistry 52, 7050-7059.

Fu, Y., Lin, H.-Y., Wisitpitthaya, S., Blessing, W.A., and Aye Y. (2014) A fluorimetric readout reporting the kinetics of nucleotide-induced human ribonucleotide reductase oligomerization.  ChemBioChem 15, 2598-2604.

Aye, Y., Long, M.J.C., and Stubbe, J. (2012) Mechanistic studies of semicarbazone triapine targeting ribonucleotide reductase in vitro and in mammalian cells: tyrosyl radical quenching not involving reactive oxygen species.  J. Biol. Chem.  287, 35768.

Aye, Y., Brignole, E.J., Long, M.J.C., Chitturulu, J., Drennan, C.L., Asturias, F.J., and Stubbe, J. (2012) Clofarabine targets the large subunit (α) of human ribonucleotide reductase in live cells by assembly into persistent hexamers.  Chem. & Biol. 19, 799.

Aye, Y. and Stubbe, J. (2011) Clofarabine 5´-Di and –Triphosphates inhibit human ribonucleotide reductase by altering the quaternary structure of its large subunit.  Proc. Natl. Acad. Sci. USA 108, 9815.